Which interactions can be studied by ITC ?

Using ITC we can follow all reactions accompanied with heat exchange. Thus the wide class of biomolecular interactions could be studied by ITC including protein-protein, protein-DNA, protein-drugs, protein-ions, DNA-ions and so on. Contrary to other methods the is no size limitations or need for molecule labeling. Nevertheless, some entropy driven reactions proceed with very low heat exchange or even without heat exchange at all. Sometimes it is possible to bypass this limitation performing ITC experiment at different temperatures.